Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs
نویسندگان
چکیده
BACKGROUND The sensitivity, specificity, and agreement of 4 diagnostic assays (SNAP canine pancreatic lipase (cPL), specific cPL (Spec cPL), VetScan cPL Rapid Test, and Precision PSL) for pancreatitis in dogs have not been directly compared. HYPOTHESIS/OBJECTIVES To determine the level of agreement among each of the 4 assays and a clinical suspicion score, level of agreement among the assays, and sensitivity and specificity of each assay in a clinically relevant patient group. ANIMALS Fifty client-owned dogs with clinical signs of gastrointestinal disease. METHODS Prospective study. History, physical examination, complete blood count, serum biochemistry, abdominal ultrasound examination, and the 4 diagnostic assays for pancreatitis were performed. Intraclass correlation coefficients (ICC) were used to determine the level of agreement between each assay and a clinical suspicion score determined by a panel of 5 board-certified veterinary internists. RESULTS The ICC between the clinical suspicion score and the 4 assays were SNAP cPL, 0.61; Spec cPL, 0.68; VetScan cPL Rapid Test, 0.68; and Precision PSL, 0.60. The sensitivities of the assays ranged from 73.9 to 100.0%, whereas the specificities were SNAP cPL, 71.1-77.8%; Spec cPL, 74.1-81.1%; VetScan cPL Rapid Test, 76.9-83.8%; and Precision PSL, 64.0-74.3%. CONCLUSIONS AND CLINICAL IMPORTANCE A good to excellent level of agreement was demonstrated among the 4 assays. The previously unreported sensitivity and specificity of the VetScan cPL Rapid Test were 73.9-83.3% and 76.9-83.8%, respectively. Results of any of the 4 diagnostic assays alone, in the absence of supporting clinical findings, are insufficient to establish a diagnosis of clinical pancreatitis in dogs.
منابع مشابه
Canine Pancreatic‐Specific Lipase Concentrations in Clinically Healthy Dogs and Dogs with Naturally Occurring Hyperadrenocorticism
BACKGROUND Specificity of canine pancreatic lipase immunoreactivity (cPLI) assays in dogs with hyperadrenocorticism (HAC) is unknown. HYPOTHESIS Results of cPLI assays differ for clinically healthy dogs and dogs with HAC. ANIMALS Seventeen healthy dogs and 20 dogs with HAC diagnosed by ACTH stimulation test results without evidence of clinical pancreatitis. METHODS Dogs were enrolled betw...
متن کاملCommon Questions from Rdvms regarding the Gastrointestinal and Immune Systems in Dogs and Cats
The canine pancreatic specific lipase is available as a SNAP cPL and as a quantitative test Spec cPL. Sensitivity of Spec cPL concentrations vary for mild pancreatitis (21%), moderate to severe pancreatitis (71%), and pancreatitis overall (64–94%). Currently, serum cPL concentration is considered the most specific serum biomarker for the diagnosis of pancreatitis in dogs. Specificities range fr...
متن کاملEvaluation of the SNAP CPL and Spec CPL Canine Pancreatic Lipase Tests for Acute Pancreatitis in Dogs Presenting with Clinical Signs of Acute Abdominal Disease
................................................................................................................................ ii LIST OF TABLES ...................................................................................................................... vi LIST OF FIGURES ...................................................................................................................
متن کاملAgreement of Serum Spec cPL with the 1,2‐o‐Dilauryl‐Rac‐Glycero Glutaric Acid‐(6′‐methylresorufin) Ester (DGGR) Lipase Assay and with Pancreatic Ultrasonography in Dogs with Suspected Pancreatitis
BACKGROUND Spec cPL is the most sensitive and specific test for diagnosing pancreatitis in dogs. Its results have not been compared to those of the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay or those of abdominal ultrasonography. OBJECTIVES To investigate agreement of Spec cPL with DGGR lipase activity and pancreatic ultrasonography in dogs with ...
متن کاملEvaluation of Canine Pancreas‐Specific Lipase Activity, Lipase Activity, and Trypsin‐Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs
BACKGROUND Diagnosis of pancreatitis in dogs is complicated by extrapancreatic disorders that can alter the results of laboratory tests. Extrapancreatic disorders can also affect the diagnosis of exocrine pancreatic insufficiency (EPI). The effects of acute kidney injury (AKI) on pancreas-specific lipase activity (Spec cPL(®) Test), serum lipase activity and trypsin-like immunoreactivity (TLI) ...
متن کامل